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Pregnant women are at increased risk of iron deficiency because iron requirements are significantly increased during pregnancy due to increased iron utilization by the developing fetus and placenta and because of blood volume expansion (69)

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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample