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Primary antibodies used included goat anti-choline acetyltransferase (ChAT, 1:1000 dilution, AB144P, Millipore) and rabbit anti-c-Fos (1:1000 dilution, 2250 S, Cell Signaling Technology)

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longer under freezer storage when documentation supports it Lyophilized powder for long-term storage -20 C freezer, dry container, minimal temperature cycling Best option for long-term retention before reconstitution Reconstituted BPC-157 2-8 C refrigerator, protected from light About 28 days as a conservative default unless supplier data says otherwise Unknown temperature history Not reliable Discard or replace rather than guessing Lyophilized BPC-157 Lasts Longer Than Reconstituted BPC-157 Most research BPC-157 is supplied as lyophilized powder

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