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These regions were chosen because of their putative role in the neural mechanisms of ME/CFS and long COVID, in particular with regard to the integration of information from various levels [33, 34], with pgACC key for emotional regulation and autonomic integration, and dACC - for cognition [35, 36]

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Mixing the sample or standard with the supplied detection reagent and GSH and incubating at room temperature for 30 minutes yields a fluorescent product which is read at 460 nm in a fluorescent plate reader with excitation at 390 nm