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Its a 15-amino-acid peptide originally derived from a protective protein naturally found in human gastric juices, and it has been studied for more than 30 years by multiple independent research groupsmost notably teams in Croatia and Taiwan

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Samples containing 100 g of heart lysate in 200 L of 50 mM HEPES buffer (pH 7.4) were treated with either buffer (control), Na 2 S (40 M) or Na 2 S 2 (40 M) for 5 min at RT