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A double reciprocal replot of k obs versus [I] did not fit to a linear relationship (unpublished data)

Laboratory and review evidence consolidated in two 2025 papers (Paschou IA et al, Endocrine 89(3):680-685, PMID 40498168

doi:10.1038/ejcn.2014.165

Interestingly, the first step in de novo pyrimidine biosynthesis mediated by CPSs occurs mainly in mitochondria via CPS1 in K-Ras/LKB1-mutant lung cancer cells 32 (Fig