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Cells were washed three times with TBS-T and incubated with secondary antibodies combining donkey anti-mouse AF555 (Thermo Fisher Scientific) and donkey anti-chicken AF647 (Thermo Fisher Scientific) diluted in TBS-T with 1% NDS for 2 hours at room temperature on an orbital shaker

The dual mechanism means more GI effects for many users

It may also help improve the function of beta cells, which produce insulin in the pancreas

The available evidence is based on few trials of small sample sizes and is thus too limited to draw firm conclusions regarding the potential benefit of long-chain PUFA supplementation in ASD management