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The protein was then incubated with a buffer consisting of 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 50 M tirzepatide, 20 g/mL Nb35, 0.03% (w/v) LMNG, 0.01% (w/v) GDN, 0.008% (w/v) CHS and 30 g/mL His-tagged TEV protease on the column overnight at 4C

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Moreover, Nataraja and Bright showed that curcumin inhibited EAE decreasing IL-12 production from macrophage/microglial cells, and by inhibition of IL-12-induced tyrosine phosphorylation of Janus kinase 2 (JAK2), tyrosine kinase (TYK2), and STAT 3 and STAT4 transcription factors leads a decrease of T cell proliferation and Th1 differentiation (Natarajan and Bright, 2002)