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The biological activity is validated using a colorimetric bioassay consisting of an engineered cell line HEK-Blue GLP-1, expressing the GLP-1R together with a CREB-inducible SEAP reporter gene ( see figure )

The research conversation around GHK-Cu has included reports of effects on tissue repair, on collagen and extracellular matrix gene expression, on antioxidant gene networks, on hair follicle biology, and on broader gene expression patterns characterized in transcriptomic studies

Source data for the Figures, Supplementary Tables, and Figures are provided as a Source Data file