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Sodium butyrate facilitates CRHR2 expression to alleviate HPA axis hyperactivity in autism-like rats induced by prenatal lipopolysaccharides through histone deacetylase inhibition

These alternatives demonstrate superior safety profiles and patient satisfaction compared to IV administration

doi: 10.1186/s40795-021-00485-5

aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF + aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 "10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 "10 M Dihexa (Harding lab) in aCSF, 10 "12 M Dihexa in aCSF or 50 ng/ml HGF + 10 " ' 2 M Dihexa in aCSF for 30 minutes at 37C with gentle rocking