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Samples were maintained at cryogenic temperatures using a closed cycle liquid He coldhead cryostat (15 K) to minimize radiation damage and maintain an inert sample environment

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An 8-week randomized double-blind clinical trial comparing GHK-Cu in nano-lipid carrier to Matrixyl 3000 showed 55.8% reduction in wrinkle volume versus control serum and 31.6% reduction versus Matrixyl 3000, with 32.8% reduction in wrinkle depth versus control

Advising a temporary stop to non-prescribed antioxidants or skin lightening supplements at least 3 days prior