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Magnesium is considered a shortfall nutrient in the diet, meaning that magnesium is underconsumed by eating the typical American diet

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Peptide mixtures from each sample were injected into an Acclaim PepMap100 C18 trap column (3 m particle size, 100 pore size, 75 m internal diameter, and 20 mm column length), which was connected to an analytical Acclaim PepMap column (2 m particle size, 100 pore size, 150 mm length, and 50 m internal diameter), with a flow rate of 200 nL/min