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To this point goes the relevance of the findings that as in the previous muscle and ligament injuries studies [19,20,21,22,31,35,36,37,47], the damaged animal exhibits spontaneous injured hind limb contracture, in particular, maintained knee flexure, inability to achieve knee extension even upon forced extension

This process requires strict control of reaction conditions, including temperature, pH value and solvent selection, to ensure the purity of the product