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Using the Connectivity Map, we identified GHK as a compound that can reverse the gene-expression signature associated with emphysematous destruction and induce expression patterns consistent with TGF pathway activation
Importantly, they also illustrate how defects at distinct levels of the UPS, substrate recognition, ubiquitin recycling or proteasome targeting, can converge on a common outcome of proteotoxic stress and neurodegeneration
For mass spectrometric analysis, the peptides were subjected to nanospray ionization (NSI) and tandem mass spectrometry (MS/MS) in the Q ExactiveTM Plus instrument, which was coupled online to the UPLC system
This intermediate is responsible for cyclization, hydrolysis, and base-exchange reactions 133
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