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NAD+ levels in human skin decline with age, and this decline affects multiple repair mechanisms simultaneously: DNA repair capacity (through PARP enzyme activity), mitochondrial energy production in skin cells, autophagy of damaged proteins, including glycated collagen, and the sirtuin-mediated preservation of collagen integrity

View at PubMed Ballatori N, Krance SM, Notenboom S, et al

Cell Concentration and Human Exposure Laboratory studies can expose cells directly to a known concentration for a defined period