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A similar strategy was used by DallAcqua and colleagues to generate human IgG1 variants with increased affinity for both mouse and human FcRn [100]

Mn predominantly enters the CNS through the faster extracellular route, where it is carried paracellularly via bulk flow to the CSF and subsequently diffuses into the brain ( via metal transporters ( 54 Mn uptake (Thompson et al., 2007) (Figure 2)

This ensures they get the right amount for their needs

Systemic effects including cognitive benefits, mood regulation, and general tissue maintenance could work with either route, though injection delivers more predictable exposure levels