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The plasmid for the non-tagged SUN2 expression was cloned by cutting the SUN2-CFP plasmid 65 with EcoRI and BamHI, blunting with Phusion DNA Polymerase (New England Biolabs, M0530), and re-ligating the plasmid thus introducing a stop codon in frame with the SUN2 sequence

A lipid panel checks your cholesterol levels specifically your LDL (bad) cholesterol, HDL (good) cholesterol, and triglycerides

This concept would be in line with our findings of COL I-triggered melanogenesis, opening the possibility that MC hyperactivity in melasma could, at least in part, result from the exposure to dermal COL I

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