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The measurements further allow a first in vivo estimate for the NADPH/NADP + ratio in the cultured yeast cytosol in the range of 0.030.3 ( Figure 1, 2 and Figure S6 )

Even if cysteine S -conjugate -lyase is responsible for the synthesis of the highly reactive and cytotoxic thiol version of cisplatin ( Figure 1 ), the activation of cisplatin into a nephrotoxin relies on the three previous steps of the classical mercapturic acid pathway involving GSTs (GSH conjugation), GT (production of CisPt-cys-glu) and dipeptidase (production of CisPt-cys

These compounds then re-enter the cell via various transporters including organic anion transport polypeptides and cystine/cysteine importers (Hinchman et al., 1998