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Glycine and serine (serine is converted to glycine for GSH synthesis) are spent making GSH, but glutamate is increased once GSH exits the cell and is transaminated by GGT, releasing glutamate into circulation

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As a consequence, GDH flux (Figure 1, ) increases [5] supplying anaplerosis as malate exits the cycle forming pyruvate which in turn supports citrate formation (Figure 2)