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Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

Technical specificatio n Highly Purified Vitamin D3 Injection The Vitamin D3 Injection is made to ensure purity and stability, providing a consistent dose in every administration

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Recommended Dosing Protocols Common bodybuilding and recovery-focused protocols include: Subcutaneous: 200500 mcg once or twice daily, near the injury site or in abdominal fat Intramuscular: 200300 mcg directly into the affected muscle, rotating sites Oral: 250500 mcg dissolved in water once daily, though bioavailability may vary Many protocols begin at the lower end, around 200 mcg, during the first week to assess tolerance