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Abnormal methylation patterns as epigenetic defects have been implicated in ASDs

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Cells were lysed in radioimmunoprecipitation assay buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate and 0.1% SDS) and protein content was measured using a standard BCA kit (Beyotime, P0009)

These consolidated sections reduce redundancy and clarify the interrelationship between oxidative stress, mitochondrial damage, and apoptosis while preserving mechanistic depth and scientific accuracy