Without adequate GSH to replenish NADPH, low NADPH levels result directly in increased ROS which can damage mitochondria, DNA, and other important cellular components [24,25]
Functions of Glutathione Powerful Detoxifier Binds to and eliminates heavy metals, toxins, and pollutants
Considering that obesity and/or insulin resistance are predominant among patients with PCOS, lifestyle interventions aimed at lessening the metabolic burden of the syndrome are often at the first-line approach 45
Then, the dishes were washed by PBS, fixed with 4% paraformaldehyde, permeabilized by 0.2% Triton X-PBS solution, and then incubated with primary antibodies (p-P38, p-P65, KEAP1, Ubiquitin, and LC3) at 4 C overnight