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To further determine the effective concentration range for the safety of TZP on the differentiation of BMSCs, we selected the concentration range with four gradient ranges of 0.5 M, 1 M, 3 M, and 5 M, and we found that in the large range of concentration gradient ranges, there was no significant difference in the osteoblast differentiation index (ALP level), as well as the osteogenic mineralization index (AR staining) (Fig