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Overall, 100 l of lysis buffer (1 mg/ml lysozyme, 10 mM Tris, and 1 mM EDTA, pH 8.0) was used to resuspend the cells and then incubated at 26C for 6 min

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Oral administration of hydrolyzed collagen alleviates pain and enhances functionality in knee osteoarthritis: results from a randomized, double-blind, placebo-controlled study