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Inhibition of key ferroptosis regulators like acyl coenzyme ACSL4, GPX4, and Nrf2 may lead to unintended consequences, including disruptions to cellular homeostasis, thereby posing potential risks to therapeutic safety

After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h