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The 16S rDNA sequencing approach, though widely used, is inherently constrained by its inability to resolve microbial identity beyond the genus level and its lack of explanatory power regarding strain-specific diversity

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Mannitol, with a cross-sectional hydrated diameter of 6.5 is a small solute that is used as a marker of total intestinal surface area available for absorption, whereas lactulose (with a larger cross-sectional diameter of 9.5 ) is used to assess the paracellular permeability through the tight junctions normalized to the total available intestinal surface [32], with results expressed as a ratio of the permeability of mannitol to lactulose

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