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The heart of a mouse freshly euthanised with isoflurane overdose was surgically removed and placed into ice-cold Tyrodes solution containing the following components (mM): 142.5 NaCl, 2.5 KCl, 0.5 MgCl 2 , 0.33 NaH 2 PO 4 , 5 glucose, 2 Na lactate, 0.1 Na pyruvate, 10 Hepes and 1.8 CaCl 2 (pH set to 7.4 with NaOH)

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