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After 24 h, the HFF-1 cells in the well plates were imaged using phase contrast microscopy (Zeiss, Axio, Germany) for growth appearance, adherence and confluence

After animals were killed by exsanguination at each time point, the livers were harvested and rinsed in ice-cold saline

ROS can reduce endothelial barrier function, facilitating lipoprotein deposition and oxidative modification of low-density lipoprotein (LDL) particles in the vessel wall [104]