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For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure

Gastroesophageal reflux disease (GERD) is a chronic condition that affects the digestive system

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All the patients were seen at least eight years after the cancer treatment, which consisted of a combination of radiotherapy and chemotherapy with or without the use of cisplatin (CDDP)