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Liposomes with cyclic RGD peptide motif triggers acute immune response in mice

The proline substitutions at positions 25, 28 and 29 reduce fibrillation propensity relative to native amylin but do not abolish it, so avoid mechanical shear, avoid leaving concentrated solution standing at room temperature, and inspect for haze, filaments or opalescence before use

For example, in the 2004 study by Philip et al, researchers shaved the sides and/or backs of healthy rats and applied a topical lotion containing TB-500 or control vehicle to each side for one week