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The trial proceeded without reported toxicity, and no lethal dose threshold (LD1) was ever established in subsequent safety testing meaning researchers administering the compound could not find a dose that caused death in even 1% of test subjects across the animal model safety work that preceded and followed the human study

As a result, skin appears dark, but glutathione helps prevent skin darkness and support skin brightening

The gradient program was run as follows: 0100% methanol, 030 min, and 100% methanol, 10 min

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