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Abbreviations MAFLD: Metabolic dysfunction-associated fatty liver disease MASH: Metabolic dysfunction-associated steatohepatitis LC-MS: Liquid chromatography-mass spectrometry UPLC-Orbitrap-MS/MS: Ultra-performance liquid chromatography coupled with Orbitrap tandem mass spectrometry ALT: Alanine transaminase AST: Aspartate transaminase TG: Triglycerides TC: Total cholesterol HDL-C: high-density lipoprotein cholesterol LDL-C: Low-density lipoprotein cholesterol HFD: High-fat diet NCD: Normal diet group GLP-1: Glucagon-like peptide-1 GIP: Glucose-dependent insulinotropic polypeptide HE: Haematoxylin and eosin ORO: Oil Red O HRI: hepatorenal index DIA: Data-independent acquisition DDA: Data-dependent acquisition PRM: Parallel reaction monitoring PPI: Protein-protein interaction M6P: D-mannose-6-phosphate References Riazi K, Azhari H, Charette JH, et al

By hitting both pathways at once, tirzepatide produces a more powerful and synergistic effect on appetite suppression and metabolic regulation