Use it to understand the routine, not to make dosing decisions
This happens because your body adapts to a lower calorie intake, your energy needs decrease as you lose weight, and factors like protein intake, sleep quality, stress, bowel regularity, and overall diet consistency start to play a larger role in your metabolic response
After incubation, the supernatant was discarded and cold ECM gel from Engelbreth-Holm-Swarm murine sarcoma diluted to 0.35 mg/mL in incubation medium (William's E medium with 0.1 M dexamethasone in DMSO, 0.5% penicillin/streptomycin, 6.25 g/L human recombinant insulin, 6.25 g/mL human transferrin, 6.25 ng/mL selenous acid, 1.25 mg/mL bovine serum albumin, 5.35 g/mL linoleic acid, 2 mM GlutaMAX, and 15 mM HEPES at pH 7.4) was applied over the cells prior to an overnight incubation at 37C
In practice, this may be someone whose acute shedding exposed pre-existing androgenetic alopecia, accelerated the visibility of patterned miniaturization or left a stable density deficit after reversible contributors were corrected
