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First, although CaF 2 and NACP nanoparticles are intrinsically cytocompatible, high fluoride loads or DMAHDM quaternary ammonium antimicrobials still display concentration-dependent cytotoxicity in 3-D spheroid cultures or human dental-pulp stem-cell assays, compromising the biosafety of the pulp and surrounding soft tissues (Wendler et al., 2021)

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