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To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h

As an aside, in a randomized, double-blind, placebo-controlled study, the addition of STS (80 mg/day, IV for 2 days before and 3 days after PCI) to the standard treatments could alleviate myocardial injury and the occurrence of short-term cardiovascular events in patients with non-ST elevation acute coronary receiving PCI [156]

Cell isolation, identification, culture, and treatment Primary rat hepatocytes were isolated from male SD rats, based on the modified method 15