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Intramuscular injections , though shorter in duration, are also invasive involving deep needle penetration into muscle tissue and can cause discomfort, soreness, or localized reactions

Functional validation (cognitive testing, synaptic marker assays) is often more practical than pharmacokinetic sampling for confirming protocol effectiveness

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Furthermore, few amounts of NADs that enter cells often become trapped by endosomes and subsequently degraded by lysosomes, considerably limiting their development and application