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Flow cytometric analysis The separated leukocytes were stained with fluorescent-conjugated antibodies purchased from BioLegend, BD Biosciences (Franklin Lakes, NJ, USA), Thermo Fisher Scientific, and Tonbo Biomedicine as follows: anti-CD3, anti-CD4, anti-CD8, anti-CD11b, anti-CD11c, anti-CD45, anti-CD45R(B220), anti-CD95, anti-CD117(c-kit), anti-CXCR5, anti-FcRI, anti-GL-7, anti-IgE, anti-Ly6C, anti-Ly6G, anti-PD-1, and anti-Siglec-F (Supplementary Table 1)